Wuxian Shi, Ph.D.
National Synchrotron Light Source
Bldg. 725A-X29
Brookhaven National Laboratory
Upton, NY 11973
Office Location: Building 535, Room 1-A108
Phone: (631) 344-2099
Fax: (631) 344-5594
Email: wushi@bnl.gov
Faculty Appointments
Case Center for Proteomics (Assistant Professor)
Center for Synchrotron Biosciences at NSLS, BNL
Academic History
| 1987 | B.S., Chemistry, University of Science and Technology of China |
| 1996 | Ph.D.,Biochemistry, Pennsylvania State University |
| 1996-2000 | Post Doctoral Research Associate. Dept. of Biochemistry, Albert Einstein College of Medicine |
Positions and Employment
| 2000-2005 | Instructor. Dept. of Biochemistry, Albert Einstein College of Medicine |
| 2006- | Assistant Professor. Center for Proteomics , Case School of Medicine |
Research
My research interest is focused on analysis of structure and function of proteins using information derived from X-ray Crystallography. In particular, I am involved in structural characterization of HIV envelop glycoprotein to assist the design of HIV antigens that can elicit broadly neutralizing antibodies, with a long term goal of developing a protective vaccine against the virus. We also have collaborations on structure based drug design against the targets implicated in malarial infection and lung cancer.
We are actively involved in New York Structural Genomics Research Consortium, where a large number of structures are determined in an effort to obtain 3-D structure information for all proteins. We provide synchrotron beamline access for protein crystallographers around the world. We also developed a high-throughput technology, in association with structure genomics, to characterize metalloproteins using X-ray absorption spectroscopy. The results are used to aid in structure determination and to create a database which will include characterization of at least one protein from each of the 5000 largest protein families and detailed structure models of metal binding sites for metalloproteins.
Web Links
New York Structural Genomics Research Consortium: http://www.nrsgrc.org
Recent Publications
- Shi W, et al., Chance MR (2003) High-throughput structural biology and proteomics. In protein chips, biochips, and proteomics: The next phase of genomics discovery, Chapter 12, pp. 299-324. Marcel Decker, NY.
- Lewandowicz A, Shi W, Evans GB, Tyler PC, Furneaux RH, Basso LA,. Santos DS, Almo SC, Schramm VL (2003) Over-the-barrier transition state analogues and crystal structure with Mycobacterium tuberculosis purine nucleoside phosphorylase. Biochemistry 42: 6057-6066.
- Singh V, Shi W, Evans GB, Tyler PC, Furneaux RH, Almo SC, Schramm VL (2004) Picomolar transition state Analogue inhibitors of human 5’-methylthioadenosine phosphorylase and x-ray structure with MT-Immucillin-A, Biochemistry 43: 9-18.
- Shi W, Ting LM, Lewandowicz A, Tyler PC, Evans GB, Furneaux RH, Kim K, Almo SC, Schramm VL (2004) Plasmodium falciparum purine nucleoside phosphorylase: Crystal Structures, immucillin inhibitors and dual catalytic function. JBC, 279: 18103-18106.
- Klein MG, Shi W, Ramagopal U., Tseng Y, Wirtz D, Kovar DR, Staiger CJ, Almo SC (2004) Structure of the actin crosslinking core of fimbrin. Structure 12: 999-1013.
- Ting LM, Shi W, Lewandowicz, A, Singh V, Mwakingwe A, Birck MR, Ringia EA, Bench G, Madrid DC, Tyler PC, Evans GB, Furneaux RH, Schramm VL, Kim K (2005) Targeting a novel Plasmodium falciparum purine recycling pathway with specific immucillins. JBC 280: 9547-54.
- Shi W, Zhan C, Manjasetty B, Marinkovic N, Sullivan M, Huang R, Chance MR (2005) Metalloproteomics – Metalloproteomics-high throughput determination of transition metal content of metalloproteins. Structure 13: 1473-1486.
- Zhan C, Fedorov EV, Shi W, Ramagopal UA, Thirumuruhan R, Manjasetty BA, Almo SC, Fiser A, Chance MR, Fedorov AA (2005) The ybeY protein from E. coli is a metalloprotein containing nickel. Acta. Cryst. F, F61: 959-963.
- Shi W, Chance MR (2006) Structural Genomics: high-throughput methods in structure determination of protein domains, in Comprehensive Medicinal Chemistry II, Volume 3: Drug Discovery Technologies, C. Hansch, Ed., Elsevier, in press.
- Manjasetty BA, Shi W, Zhan C, Fiser A, Chance MR (2006) High throughput approach to protein structure analysis. In Genetic Engineering: Principles and Methods. Chapter 28, J.K Setlow (ed.), Springer, New York, in press.
- Singh V, Shi W, Almo SC, Evans GB, Furneaux RH, Tyler PC, Painter GF, Lenz DH, Mee S, Zheng R, Schramm VL (2006) Structure and Inhibition of a Quorum Sensing tartget from Streptococus pneumoniae. Biochemistry, 45(43):12929-41.
- Shi W, et al., Chance MR (2006) Beamline X29: a novel undulator source for X-ray crystallography. J. Synchrotron Radiation, 13:365-372.