Rob M. Ewing, Ph.D.
Case Western Reserve University
Center for Proteomics
10900 Euclid Ave., BRB 936
Cleveland, OH 44106-4988
Phone: (216) 368-4380
Email: rob.ewing@case.edu
Faculty Appointments
Case Center for Proteomics (Assistant Professor)
Department of Genetics (secondary)
Academic History
| 1999-2001 | Post-doctoral fellow, Carnegie Institution of Washington/Stanford University, USA |
| 1997-1999 | Post-doctoral fellow, CNRS, Marseilles, France |
| 1996 | Ph.D, Plant molecular genetics, University of Oxford, UK. |
Positions and Honors
| 2006 | Co-founder, Infochromics, Toronto, Canada |
| 2003-2005 | Group leader, Bioinformatics, MDS Proteomics, Toronto, Canada |
| 2001-2003 | Senior bioinformatics scientist, Incyte Genomics, Palo Alto, USA |
Research
I have two principal research areas of interest:
- Development of a combined proteomics/computational approach to mapping protein-protein interaction networks and understanding how perturbations to those networks impact cellular function.
- Development of computational methods and resources for quantitative LC/MS shotgun proteomics
Over the last few years, several studies have generated (albeit partial and noisy) snapshots of protein-protein interaction networks in model organisms. These datasets have been analyzed in many ways and have provided insights into protein function and network topology. Our interest is to focus on specific sub-networks and to understand how cellular perturbations (mutation, drug, allelic variation etc) impact network function.
Relative quantitation of peptides and proteins across multiple samples is a fundamental technology for systems biology and biomarker discovery. Several methodologies have been developed including 2D-DIGE and isotope labelling for shotgun LC/MS proteomics. Our specific interest is in the development of computational methods and software for automating label-free LC/MS proteomics. We are also interested in developing repositories for peptide and protein quantitations that would enable global profiling of protein expression across cell-types, tissues and organisms.
Recent Publications
- Ewing RM, Topaloglou T, Figeys D, et al. (2007) Large-scale mapping of human protein-protein interactions by mass spectrometry Molecular Systems Biology 3:89
- Moran MF, Tong J, Taylor P, Ewing RM. (2006) Emerging applications for phospho-proteomics in cancer molecular therapeutics. Biochim. Biophys. Acta. Rev. Cancer 1766(2):230-41
- Bihan TL, Goh T, Stewart II, Salter AM, Bukhman Y, Dharsee M, Ewing RM. & Wieniewski JR. (2006) Differential Analysis of Membrane Proteins in Mouse Fore- and Hindbrain Using a Label-free Approach. Journal of Proteome Research 5(10):2701-10