Tissue Culture and Hybridoma Core Facility
Visual Sciences Research Center
Institute of Pathology, The VSRC Consolidated Laboratory Space
Ram H. Nagaraj, PhD, Facility Director
Contact:
Serves:
Overview
The Tissue Culture (TCH) Module provides visual science investigators with facilities and technical assistance for the maintenance and propagation of ocular and other cell types. The Hybridoma division is involved in the development of monoclonal antibodies to proteins and other antigens of relevance to ocular disease. The broad functions of the Tissue Culture Module are 1) isolation of primary cells from ocular tissue; 2) maintenance and passage of established cell lines, including storage; 3) large scale amplification of cell lines; 4) transient transfection of cells; 4) generation of stably transfected cell lines; 5) training in growth and maintenance of cells; and 6) strategic advice in planning experiments and determining their feasibility. Specialized cell types currently stored include human and bovine retinal capillary endothelial cells, human and bovine retinal capillary pericytes, a rat retinal Muller cell line, a human retinal pigmented epithelial cell line, human and rabbit corneal epithelial cells, a rat retinal ganglion cell line, human umbilical vein and aortic endothelial cells, and mouse and human lens epithelial cells and cell lines.
Page last updated: October, 2007
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