Cystic Fibrosis Inflammatory Mediator Core
Cystic Fibrosis Center, Pediatric Pulmonology
Biomedical Research Building (BRB) 8th Floor
Tracey Bonfield, Facility Director
Contact:
Serves: Investigators studying cystic fibrosis-related processes
Overview
The Inflammatory Mediator Core laboratory performs assays of inflammatory mediators, cytokines and intracellular second messengers. We also utilize assays for normalization of results on human and murine BAL samples, culture media, blood samples, and sputum samples using urea, albumin or other proteins. We provide the availability to evaluate of “inflammatory mediators” at the level of gene expression, secretion and function. Common assays, but not all inclusive to our abilities include human cytokines Eotaxin, IL-5, IL-1, IL-6, IL-8, IL-17, GM-CSF, and murine cytokines including IP-10, M-CSF, MIP-2, KC, TNF-alpha, IL-1beta, and IL-6. Also routinely measured are eicosanoids, cyclic nucleotides, nuclear transcription factors and various murine and human growth hormones. Secreted and intracellular cytokines can be measured using both a solid phase ELISA and fluid phase multi-analyte analyzer, Luminex Platform. Transcriptional regulation can also be done using the multi-analyte analyzer. The use of the Luminex multi-analyte analyzer allows for the simultaneous analysis of multiple cytokines, transcription factors, lipids and other inflammatory mediators from individual samples resulting in more accurate direct comparisons of cytokine expression profiles, faster processing of assays, and a lower cost per assay. We also provide services for development of multi-analyte assays if current analytes- are not commercially available.
Page last updated: October, 2007
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216-368-4558
