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THE DETAILS...
“Navigating the Cellular Landscape with New Optical Probes, Imaging Strategies, and
"Emerging visualization technologies are playing an increasingly important role cell biology, capturing processes from the level of whole organisms to single molecules. Recent developments in probes, techniques, microscopes, and quantification are dramatically expanding the areas of productive imaging. Photoactivatable fluorescent proteins (PA-FPs) allow selected populations of proteins to be pulse-labeled and tracked over time. Used for in cellulo pulse chase experiments, the PA-FPs have helped clarify mechanisms for biogenesis, targeting, and, maintenance of organelles as separate identities within cells. PA-FPs have further permitted the development of single molecule-based super-resolution (SR) imaging, which dramatically improves the spatial resolution of light microscopy by over an order of magnitude (~10-20 nm resolution). Single molecule SR imaging offers exciting possibilities for obtaining molecule-scale information on biological events occurring at variable time scales. Among the biological questions addressed are how cells crawl; how cells destroy damaged components; how single molecules move and arrange themselves on the cell surface; and how subunits of receptor complexes organize stoichiometrically.”
Date: Jan. 16, 2013
Time: 1:30 PM-2:30 PM
Location: WRB-1413
Speaker(s):
Jennifer Lippincott-Schwartz, Ph.D. Chief, Section on Organelle Biology Cell Biology and Metabolism Branch NICHD, NIH, Bethesda, MD
Contact Information:
Denise Miller
Email: dmm192@case.edu
This is a private event.
Sponsored by: Program in Cell Biology
