Ram H. Nagaraj, Ph.D.

Recent Publications :

• Biswas, A., Goshe, J., Miller, A., Santhoshkumar, P., Luckey, C., Bhat, M.B. and Nagaraj, R.H. (2007) Paradoxical effects of substitution and deletion mutation of Arg56 on the structure and chaperone function of human alphaB-crystallin. Biochemistry 46, 1117-27.

• Puttaiah, S., Biswas, A., Staniszewska, M. and Nagaraj, R.H. (2007) Methylglyoxal inhibits glycation-mediated loss in chaperone function and synthesis of pentosidine in alpha-crystallin. Exp Eye Res 84, 914-21.

• Staniszewska, M. and Nagaraj, R.H. (2007) Detection of kynurenine modifications in proteins using a monoclonal antibody. J Immunol Methods 324, 63-73.

• Staniszewska, M.M. and Nagaraj, R.H. (2006) Upregulation of glyoxalase I fails to normalize methylglyoxal levels: a possible mechanism for biochemical changes in diabetic mouse lenses. Mol Cell Biochem 288, 29-36.

• Padival, S. and Nagaraj, R.H. (2006) Pyridoxamine inhibits maillard reactions in diabetic rat lenses. Ophthalmic Res 38, 294-302.

• Miller, A.G., Smith, D.G., Bhat, M. and Nagaraj, R.H. (2006) Glyoxalase I is critical for human retinal capillary pericyte survival under hyperglycemic conditions. J Biol Chem 281, 11864-71.

• Biswas, A., Miller, A., Oya-Ito, T., Santhoshkumar, P., Bhat, M. and Nagaraj, R.H. (2006) Effect of site-directed mutagenesis of methylglyoxal-modifiable arginine residues on the structure and chaperone function of human alphaA-crystallin. Biochemistry 45, 4569-77.

• Oya-Ito, T., Liu, B.F. and Nagaraj, R.H. (2006) Effect of methylglyoxal modification and phosphorylation on the chaperone and anti-apoptotic properties of heat shock protein 27. J Cell Biochem 99, 279-91.

• Puttaiah, S., Zhang, Y., Pilch, H.A., Pfahler, C., Oya-Ito, T., Sayre, L.M. and Nagaraj, R.H. (2006) Detection of dideoxyosone intermediates of glycation using a monoclonal antibody: characterization of major epitope structures. Arch Biochem Biophys 446, 186-96.

• Puttaiah, S., Zhang, Y., Pilch, H.A., Pfahler, C., Oya-Ito, T., Sayre, L.M. and Nagaraj, R.H. (2006) Detection of dideoxyosone intermediates of glycation using a monoclonal antibody: characterization of major epitope structures. Arch Biochem Biophys 446, 186-96.

• Nagaraj, R.H., Oya-Ito, T., Bhat, M. and Liu, B. (2005) Dicarbonyl stress and apoptosis of vascular cells: prevention by alphaB-crystallin. Ann N Y Acad Sci 1043, 158-65.

• Staniszewska, M.M. and Nagaraj, R.H. (2005) 3-hydroxykynurenine-mediated modification of human lens proteins: structure determination of a major modification using a monoclonal antibody. J Biol Chem 280, 22154-64.

• Liu, B., Bhat, M., Padival, A.K., Smith, D.G. and Nagaraj, R.H. (2004) Effect of dicarbonyl modification of fibronectin on retinal capillary pericytes. Invest Ophthalmol Vis Sci 45, 1983-95.

• Liu, B., Bhat, M. and Nagaraj, R.H. (2004) AlphaB-crystallin inhibits glucose-induced apoptosis in vascular endothelial cells. Biochem Biophys Res Commun 321, 254-8.

• Padival, A.K., Crabb, J.W. and Nagaraj, R.H. (2003) Methylglyoxal modifies heat shock protein 27 in glomerular mesangial cells. FEBS Lett 551, 113-8.

• Nagaraj, R.H., Oya-Ito, T., Padayatti, P.S., Kumar, R., Mehta, S., West, K., Levison, B., Sun, J., Crabb, J.W. and Padival, A.K. (2003) Enhancement of chaperone function of alpha-crystallin by methylglyoxal modification. Biochemistry 42, 10746-55.

• Mathys, K.C., Ponnampalam, S.N., Padival, S. and Nagaraj, R.H. (2002) Semicarbazide-sensitive amine oxidase in aortic smooth muscle cells mediates synthesis of a methylglyoxal-AGE: implications for vascular complications in diabetes. Biochem Biophys Res Commun 297, 863-9.

• Nagaraj, R.H., Sarkar, P., Mally, A., Biemel, K.M., Lederer, M.O. and Padayatti, P.S. (2002) Effect of pyridoxamine on chemical modification of proteins by carbonyls in diabetic rats: characterization of a major product from the reaction of pyridoxamine and methylglyoxal. Arch Biochem Biophys 402, 110-9.