| Week |
Lecture |
Lecturer |
Reference |
| I |
- INTRODUCTION TO TOXICOLOGY
- a. Course organization
- b. Definitions and scope
|
McCoy |
- Chap. 1 Hodgson
- and 6 Gibson
|
| II |
- INTRODUCTION TO XENOBIOTIC METABOLISM
- a. Cellular and subcellular fractionation
- b. Enzyme kinetics
- c. Enzyme purification
- d. Marker enzymes
|
McCoy |
(Handouts) |
| III |
- BIOLOGICAL OXIDATION I
- a. Definition of oxidative processes
- b. Redox reactions
- c. Enzyme classes catalyzing biological
oxidations
|
McCoy |
Chap. 3 Hodgson |
| IV |
- BIOLOGICAL OXIDATION II
- a. Role of oxygen in biological oxidations
- b. Biological reduction of oxygen
- c. Enzymes which utilize reduced forms of oxygen
- d. Role of biological oxidation in xenobiotic
metabolism
|
McCoy |
Chap. 1 Gibson |
| V |
- PHASE I REACTIONS; CYTOCHROME P-450
- a. Endoplasmic reticulum electron transport
components
- b. Reactions catalyzed by the enzyme system
- c. Mechanism and regulation of the enzyme
activity
- d. P450 as an enzyme family
|
McCoy |
Chap. 4 Hodgson and 2 Gibson |
| VI |
- PHASE I REACTIONS: OTHER BIOLOGICAL OXIDASES
- a. Flavin mono-oxygenase - reactions and tissue
distribution
- b. Aldehyde oxidase - reactions catalyzed
- c. Alcohol dehydrogenase - reactions catalyzed
- d. Prostaglandin syntheses - reactions catalyzed
and
- role in extrahepatic oxidations
|
McCoy |
Chap. 3 Hodgson and 1, 2 Gibson |
| VII |
EXAM I |
McCoy |
|
| VIII |
- MODIFICATION OF METABOLISM - I
- a. Nutritional effects
- b. Physiological effects
- c. Genetic effects
- d. Chemical an environmental effects
|
McCoy |
Chap. 6 Hodgson and 3 Gibson |
- IX
|
- PHASE II REACTIONS
- a. Role of glutathione in detoxification
- b. Glutathione transferases
- c. Other enzymes involved in glutathione
metabolism
- d. Epoxide hydrase
- e. Enzymes involved in conjugation reactions
|
Mukhtar |
Chap. 3 Hodgson and 2 Gibson |
| X |
- FUNDAMENTALS OF BIOLOGICAL MEMBRANE LIPID
PEROXIDATION
- a. Importance of lipid peroxidation in toxicology
- b. Chemistry of lipid peroxidation: Initiation by
carbon-centered and by oxygen-centered radicals;
role of iron
- c. Propagation of lipid peroxidation; importance
of iron in autocatalytic spread of lipid
peroxidation
- d. Termination of lipid peroxidation; biological
consequences of termination
- e. Role of vitamin E and glutathione in
protection against lipid peroxidation
- f. Role of superoxide dismutase and glutathione
peroxidases in protection against lipid
peroxidation
- g. Sequestration of iron
- d. Definition of oxidative stress
- h. Xenobiotics (drugs, poisons, solvents, etc.)
- i. Iron overload
- j. Lipid peroxidation initiated by partially
reduced species of oxygen (superoxide and
hydroxyl radicals).
|
Mukhtar |
Chap. 3 and 8 Hodgson Handouts - book chapter
|
| XI |
- MODIFICATION OF METABOLISM - II
- a. Effects of activators, inducers and inhibitors
|
McCoy |
Chap. 6 Hodgson, 4 and 5 Gibson |
| XII |
EXAMPLES OF TOXIC ACTION I |
McCoy |
Chap. 7 Hodgson |
| XIII |
EXAMPLES OF TOXIC ACTION II |
McCoy |
Chap. 8 Hodgson |
| XIV |
EXAM II |
McCoy |
|
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