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Case Western Reserve University Researchers Turn Urine into Research Tools

Breakthrough non-invasive technique generates stem cells to study Down syndrome

March 28, 2017

One of the biggest challenges in studying Down syndrome is finding the right research model. Animals and established cell lines are limited in their ability to mimic human disease, and results don’t always translate to patient populations. Stem cells hold enormous potential as research tools that can be collected directly from patients and grown into innumerable cell types. But harvesting stem cells can be tricky and invasive—a tough sell to institutional review boards when dealing with children or patients with intellectual disability.

Now, researchers from Case Western Reserve University School of Medicine have developed a breakthrough technique to harvest cells directly from urine, and grow them into durable, clinically relevant stem cells to study Down syndrome. The non-invasive technique, described in the journal STEM CELLS Translational Medicine, helps creates urgently needed research models for Down syndrome, and can also be used to model other neurologic conditions.

“For the first time, we were able to create induced pluripotent stem cells, or iPSCs, of persons with Down syndrome by cells obtained from urine samples,” said Alberto Costa, MD, PhD, study lead and professor of pediatrics and psychiatry at Case Western Reserve University School of Medicine. “Our methods represent a significant improvement in iPSC technology, and should be an important step toward the development of human cell-based platforms that can be used to test new medications designed to improve the quality of life of people with Down syndrome.”

Costa’s technique overcomes ethical challenges related to harvesting stem cells that have previously been collected via skin biopsies. According to the paper, “Although only mildly invasive, there have been anecdotal reports that a few IRBs or ethical committees have rejected research proposals for wide-scale use of skin biopsies in individuals with Down syndrome. There has also been anecdotal reports of a significant percentage of persons with Down syndrome or their parents/guardians rejecting the procedure, which has limited the establishment of Down syndrome iPSC banks.” The new technique allows researchers to more easily build collections of stem cells for use in future studies.

Stem cells generated from urine also appear to be more stable than those generated via skin biopsies. Since skin cells are exposed to sunlight, they often have DNA damage caused by ultraviolet radiation. The stem cells generated by Costa’s team did not involve cells exposed to the sun, and were manipulated using methods designed to limit DNA damage. The resultant iPSCs can be reliably grown into many cell types relevant for Down syndrome research, including neurons and heart cells. The study is the first to successfully generate such cells from urine cultures.

In total, Costa’s team generated iPSCs from urine donated by 10 individuals with Down syndrome that can be used by his team and other researchers interested in modeling Down syndrome disease states. The new technique can also be used to generate countless additional cell lines from Down syndrome patients or other vulnerable populations. The new research models offer an ethically sound, clinically relevant, and highly translatable means to study human disease.

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Funding for the study was provided by grants from ALANA USA Foundation (Contracts 124124 and 200381), the Alana CWRU/MIT Collaborative Fund, a grant from the Ohio Department of Developmental Disabilities, and charitable contributions from the Awakening Angels Foundation. Bruna Zampieri, PhD was supported by a Postdoctoral Fellowship from the Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil (CNPq/MCTI, 202237/2014-1).

For more information about Case Western Reserve University School of Medicine, please visit: http://case.edu/medicine.

 

Founded in 1843, Case Western Reserve University School of Medicine is the largest medical research institution in Ohio and is among the nation's top medical schools for research funding from the National Institutes of Health. The School of Medicine is recognized throughout the international medical community for outstanding achievements in teaching. The School's innovative and pioneering Western Reserve2 curriculum interweaves four themes--research and scholarship, clinical mastery, leadership, and civic professionalism--to prepare students for the practice of evidence-based medicine in the rapidly changing health care environment of the 21st century. Nine Nobel Laureates have been affiliated with the School of Medicine.

Annually, the School of Medicine trains more than 800 MD and MD/PhD students and ranks in the top 25 among U.S. research-oriented medical schools as designated by U.S. News & World Report's "Guide to Graduate Education."

The School of Medicine is affiliated with University Hospitals Cleveland Medical Center, MetroHealth Medical Center, the Louis Stokes Cleveland Department of Veterans Affairs Medical Center, and the Cleveland Clinic, with which it established the Cleveland Clinic Lerner College of Medicine of Case Western Reserve University in 2002. case.edu/medicine.


Media Contact(s):

Marc Kaplan
Associate Dean, Marketing and Communications
The School of Medicine
Case Western Reserve University
Office: 216-368-4692
Marc.Kaplan@case.edu

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