Light Microscopy Imaging Core Facilities

The Light Microscopy Imaging Core Facilities on the Case Western campus are shared resources that offer a wide range of imaging services to the research community. Our facilities are committed to enhancing and expanding collaborative capabilities of research in northern Ohio. Imaging techniques available through the cores include:

  • standard transmitted light microscopy:
    • brightfield
    • darkfield
    • phase contrast
    • interference contrast
    • polarization
    • fluorescence
  • live-cell long term imaging
  • confocal
  • multi-photon
  • deconvolution
  • TIRF
  • super resolution
  • slide scanning (brightfield and fluorescence)

Our range of instruments allows for most types of modern imaging techniques from basic widefield imaging to very high spatial and temporal resolution imaging of fixed and live cells, tissues or small organisms.
Image processing and analysis capabilities include image:

  • quantification
  • co-localization analysis
  • deconvolution
  • 3D reconstruction
  • stereology

Those microscopes are often used for analyzing human and animal cells under pathological conditions such as cancer, neuronal degeneration, and metabolic disorders. By allowing high-resolution and dynamic analysis of biological tissues under pathological status, this core supports translational research. An additional application includes optimization of cell-based fluorescence assays for drug screening. Moreover, the slide scanning capability allows completely automated, high-throughput analysis of fixed human pathology specimens while the image processing capabilities allow unbiased comparison of samples from healthy and diseased tissues.

Location:

  • W510 and E632

Website:

Contacts: