Mass Spectrometry and other major equipment:

The Proteomics Core laboratory has a full complement of mass spectrometry and proteomics facilities available including:

  • Thermo-Finnigan Fourier Transform LTQ mass spectrometer with ppm resolution for top-down and bottom-up proteomics capabilities operated with Dionex capillary LC system; a 96-well autosampler and switcher is utilized with this system.
  • Thermo Scientific LTQ-OT-11000 LTQ Orbitrap ETD with a NanoAquity UPLC system (Waters).
  • Thermo Scientific LTQ Orbitrap Velos with NanoACQUITY UPLC system (Waters).
  • 2 Thermo Scientific Velos Elite with NanoACQUITY UPLC systems.
  • Thermo Scientific TSQ Quantum Ultra triple quadropole mass spectrometer with NanoACQUITY UPLC system (Waters).
  • GE/Amersham 2-D gel DIGE system with robotic spot picking.
  • Beckman Biomec FX robotic liquid handling systems is available with 96-well head and SPAN-8 flexible arm. Numerous ALPs are available including heating/cooling and vacuum filtration.

Other major equipment includes: Protein solutions light scattering instrument, time-resolved fluorescence using CCD detection, FTIR, and Uv-Vis spectrometers

Computational Facilities:

  • Proteus Module Software from Genologics on a 2 TB Dell PowerEdge server for automated data acquisition, analysis.
  • Clinical Barcoding System
  • Data acquisition computers attached to mass spectrometers, scanners, Beckman BioMex FX robot, HPLCs and other analytical equipments; Data analysis software:
    1. Elucidator/Rosetta
    2. Mascot
    3. Spotfire
    4. DeCyder
    5. Ingenuity Pathway Analysis (IPA)
    6. Scaffold
    7. X! Tandem
    8. Sequest
    9. ProtMapMS
    10. Progenesis
  • Dedicated, remote access data analysis workstations including 2 workstations with BioWorks for mass spectrometry (MS) database searches, 2 workstations with DeCyder 2D software for 2D-DIGE data analyses.
  • High-end, dual-processor, multi-threaded server for Mascot MS database search engine.
  • Closed-circuit camera system for trouble shooting and user instructions of Mass Spectrometers with computer recording capability.